Table 1 PCR primers, real-time PCR primers and RNA probe sequence of mouse c-kit gene

PCR primers

Exons 10-12

Sense 5′ -TGGGGATCATTGTGATGGT-3′

Anti-sense 5′-ATGGCAGCATCCGACTTAAT-3′

Exons 18-20

Sense 5′ - CCTCTGGGAGCTCTTCTCCT-3′

Anti-sense 5′- GCTGTCCGAGATCTGCTTCT-3′

Real-time PCR primers

Exon 7-8

AACGTTTACGT GAACACAAAACCAG

Exon 20-21

GCACCAAGCACATTTACTCCAACTT

Exon 21+

CTGATATGTTGTCCAACTGTTGACA

Exons 10-12 probe (extracellular domain)

ATGGCAGCATCCGACTTAATCAAGCCATATGCAGTGGCCTCAACGACC

TTCCCGAAGGCACCAGCTCCCAATGTCTTTCCAAAACTCAGCCTGTTTC

TGGGAAACTCCCATTTGTGATCATAAGGAAGTTGCGTCGGGTCTATGT

AAACATAATTGTTTCCATTTATCTCCTCGACAACCTTCCATTGTACTTC

ATACATGGGTTTCTGCAAATATTTGTAGGTGAGCAC CATCACAATG

ATCCCCAT

Exons 18-20 probe (intracellular domain)

GCTGTCCGAGATCTGCTTCTCAATAAGTTGGACAACCTGCTTGAATGT

TGGCCTTTTCAAGGGGTCAGCGTCCCAGCAAGTCTTCATGAC

GTCATACATTTCGGCAGGCG CGTGCTCCGG GCTGACCATC

CGGAAGCCTTCCTTGATCATCTTGTAGAACTTGGAGTCGACCGGCATC

CCTGGGTAGGGGCTGCTTCCTAAGGAGAAGAGCTCCCAGAGG