Figure 7
From: c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
Immunofluorescence staining of Kit expressed in c18-4 and CRL-2053. Images were obtained with ZEISS Axioplan 2 imaging system and Spot 4.7/SpotAdvanced software (Magnification × 400). Kit positive cells were stained with green or red fluorescein (arrow). The cell nuclei was counterstained with 4′,6-diamidino-2-phenylindole. Images were merged with Photoshop CS4. (A) FITC labeled anti-Kit extracellular domain in c18-4. (B) Alexa Fluo 594 labeled anti-Kit intracellular domain in c18-4. (C) FITC labeled anti-Kit extracellular domain in CRL-2053. (D) Alexa Fluo 594 labeled anti-Kit intracellular domain in CRL-2053.