Figure 3

c-kit transcripts before and after differentiation of SSCs. (A) Full-length transcript. This transcript was composed of 21 exons and with a size about 5.2 kb. A 2.1 kb 3′ UTR region located at exon 21 of this transcript and it was expressed by SSCs before and after differentiation. Green triangles on this figure represented 3 groups of c-kit primers that hit exons 7-8, exons 20-21 and exon 21 respectively. (B) Short 3′ UTR transcript (4 kb). This transcript was composed of 21 exons with 966 bp long 3’ UTR. (C) Short transcript (2.7 kb) started from intron 9 of the full-length transcript. (D) The truncated transcript (1.5 kb). It represented a group of transcripts that started from different intron or exon of c-kit gene, the Intron/Exon X means the beginning of these transcripts. We found some of these transcripts started from exon 13, exon 15 and exon 17 of the c-kit gene respectively. All these truncated transcripts we found containing a conserved domain from exon 17 to exon 21.