Figure 4

RT-PCR analysis of DGKθ expression in mouse embryo. The DGKθ mRNA levels were determined by semiquantitative RT-PCR. The number of amplification cycles for DGKθ and GAPDH were 35 and 28, respectively. The amount of template for each PCR was normalized to the expression level of GAPDH. (A) Relative expression of DGKθ in diverse organs at E14.5. The values (mean ± SEM, n = 4) represent the relative level of expression in the whole brain compared with the lung, liver, intestine, or kidney. ** indicates P < 0.01 and * indicates P < 0.05 (B) Relative expression of DGKθ at each developmental stage in the epidermis of the dorsal skin. The amount of template for each PCR was normalized to the expression level of GAPDH. The values (mean ± SEM, n = 4) represent the relative level of expression at E14.5. * indicates P < 0.05 compared with E17.5 or adulthood skin.