Figure 8

Zic2a and Zic2b depletion reduces RA signaling levels. Tg(12xRARE-ef1a:eGFP)^sk71 transgenic embryos were used to assay the level of RA signaling. To increase sensitivity of transgene detection, in situ hybridization for eGFP was employed. Control p53MO-injected embryos (A, B) were compared with Zic2a;2b;p53MO-injected embryos (C, D). Quantification of reduction in eGFP in Zic2a;2b;p53 morphants is displayed graphically with reduced expression in 75 ± 2% of embryos (p-value = 0.0004; unpaired t-test). Cyp26a1 (F) and Aldh1a2 (G) morpholinos were also injected into Tg(12xRARE-ef1a:eGFP)^sk71 embryos to compare effects with those obtained using Zic morpholinos. Images are of 26 hpf embryos in lateral (A, C, F, G) and dorsal (B, D) views with anterior to left and top, respectively.