Figure 10

Vagal neuron domain in Zic2a and Zic2b morpholino injected embryos is partially rescued with low doses of RA. Dorsal views of the vagal motor neurons in 48 hpf Tg(isl1:eGFP) embryos that were injected with p53 morpholino (A-C) or Zic2a;Zic2b and p53 morpholinos (D-F), and treated with 1% DMSO (A, D), 1 nM RA (B, E), or 1 μM DEAB (C, F). Quantification of vagal motor neuron domain area (G) demonstrates that Zic2a/2b depletion causes a statistically significant reduction in domain size, which is rescued by treatment with retinoic acid. Quantification of vagal neuron domain length (H) gives similar results, with a statistically significant reduction upon Zic2 depletion, which could again be counteracted by retinoic acid addition. Statistical significance calculated by ANOVA and Tukey’s HSD post-hoc test (*p-value ≤ 0.01).