Skip to main content

Table 4 Effect of inducing levels of porcine insulin on [ 3 H] Ouabain binding to the plasma-vitelline membrane of the Rana Pipiens oocyte in meiotic prophase arrest 1

From: Plasma membrane events associated with the meiotic divisions in the amphibian oocyte: insights into the evolution of insulin transduction systems and cell signaling

Additions

[3H] Ouabain binding Kd2

Ouabain binding capacity fmols/oocyte

None

2.0 ± 0.19 × 10-6 M

80 ± 4

 

3.2 ± 0.12 × 10-8 M

15 ± 2

Insulin, 10 μM

4.9 ± 0.23 × 10-8 M

53 ± 3.3

  1. 1Means ± SD (N = 3 frogs).
  2. 2In the absence of insulin, ouabain binding was biphasic, with high and low affinity sites. In the presence of insulin the low affinity sites disappeared and the binding capacity of high affinity sites increased.
Back to article page

BMC Developmental Biology

ISSN: 1471-213X

Contact us