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Table 1 Comparison of porcine insulin and progesterone on the meiotic response using denuded Rana pipiens Oocytes

From: Plasma membrane events associated with the meiotic divisions in the amphibian oocyte: insights into the evolution of insulin transduction systems and cell signaling

Additions1

Meiotic response

Meiotic response

 

% Nuclear breakdown

% 2nd Metaphase arrest

None

0 (6)2

0 (6)

Insulin, 10 μM

74 ± 5.2 (4)

0 (4)

Progesterone, 0.32 μM

97 ± 3.8 (4)

29 ± 3.8 (4)

Insulin , 10 μM + Progesterone, 0.32μM

99 ± 2 (4)

96 ± 1.1 (4)

  1. 1Incubated in modified Ringer’s solution for 24 h at 20–22ºC. Experiments were carried out in February-March. Values shown are the maximal response.
  2. 2Mean ± SD (Number of frogs).
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BMC Developmental Biology

ISSN: 1471-213X

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