Figure 1

PKCδ expression at E8.5 and E9.5. A-C, lack of signal due to endogenous β-galactosidase was confirmed by applying the same LacZ staining protocols on wild type embryos (controls). D, at E8.5, X-Gal staining is detected in the yolk sac and primitive heart (ys and ph, respectively). E-H, at E9.5, trigeminal (V) neural crest tissue (tnc), rostral extremity of the notochord (not), dorsal aorta (dor), somites (som), pericardium (per) and developing heart all showed LacZ signal. In the latter, reporter activity was detected at the walls of the primitive ventricle (ven), atrium (atr) and truncus arteriosus (tru). I-L, Antibody staining of 4 μm E.9.5 embryo sections confirmed the expression of PKCδ in heart and somites. E9.5 PKCδ deficient embryo sections (J) were used as a negative control for the antibody.