Figure 4

IHC analysis of H2B-GFP expression in pulse-chase experiments with compound hnRNP-rtTA/tetO-H2B-GFP mice. A) Untreated compound animals do not express nuclear H2B-GFP. B) After 7 days of doxycycline treatment in the drinking water (pulse) the vast majority of esophageal cells are positive for nuclear GFP expression. After the doxyxycline pulse, mice were chased (no dox in drinking water) and analyzed at 5 (C), 7 (D), 10 (E) and 12 days (F). Images were taken at 60x magnification. (G) The average number of H2B-GFP expressing basal cells of animals chased for 0, 5, 7, 10, 12 and 14 days is displayed. Displayed are the average percentages of H2B-GFP expressing cells from the total population of basal esophageal cells (±standard deviation).