Figure 6
From: In vivo role of different domains and of phosphorylation in the transcription factor Nkx2-1
Expression of E-cadherin, Ksp-cadherin and ZO-1 in developing thyroid. Transversal sections of thyroid gland of wild type (A-D) and PM/PM (E-H) E14.5 embryos. Double immunofluorescent staining of Nkx2-1 and E-cadherin in wild type (A and B) and in PM/PM (E and F) mouse thyroids. E-cadherin is present on the plasma membrane of both wild type and PM/PM thyrocytes. Thyrocytes are identified by the nuclear staining of Nkx2-1 (A and E). Adjacent sections were stained by immunofluorescence with anti Ksp-cadherin (C and G) and anti ZO-1 (E and H) antibodies. The expression of Ksp-cadherin in PM/PM mouse thyroids(G) is significantly reduced with respect to wild type (C). ZO-1 is concentrated in spots on the membrane of some cells that possibly surround small lumina (D, arrows) while in PM/PM thyrocytes there is only a diffuse ZO-1 staining (H). Bar 10 μm.