Table 2 Description of RT-PCR or PCR primers for examined genes
From: Method to isolate polyribosomal mRNA from scarce samples such as mammalian oocytes and early embryos
Gene name | Gene symbol | Primer sequences 5'-3' | Amplicon size (bp) | Annealing/Melting temp. (°C) | Accession number |
|---|---|---|---|---|---|
Cyclin B1 | CCNB1 | F: ACC TGG CAA AGA ATG TGG TC R: GCT GTG CTA GAG TGC TGA TCT TAG | 108 | 60/80 | NM_001045872 |
Cyclin Dependant kinase 1 | CDK1 | F: GAT CCT GCC AAA CGA ATT TCT GGC R: TCT GCT CTT GAC ACA ACA CAG GGA | 121 | 60/78 | NM_174016 |
Oocyte maturation factor MOS | MOS | F: CAA AGC ATT GTG CAC TTG GAC CTC R: TGG GTG TAA CAG GCT CTC CTT TGA | 190 | 60/89 | XM_590874 |
Actin beta | ACTB | F: CGCCATGGATGATGATATTG R: GGTCATCTTCTCACGGTTGG | 363 | 60/N/A | NM_173979 |