Figure 3
From: Method to isolate polyribosomal mRNA from scarce samples such as mammalian oocytes and early embryos
Distribution of Drosophila SL2 polyribosomes in sucrose density gradient fractions as detected by UV absorbance. A) Untreated cytoplasmic extract; B) Cytoplasmic extract treated with 3.37% formaldehyde and quenched with 1 M Tris. Ribosomal subunits (40S and 60S), monoribosomes (80S), polyribosomes and corresponding fraction numbers are indicated. Top of gradient corresponds to fraction 1.