Figure 4

Comparison of LacZ-Positive Cells in a P0-Cre/R26R Embryo and PDGFRα Protein Expression in a Wild-Type Embryo. E9.5 P0-Cre/R26R embryo (a, c) and wild-type embryo (b, d) (n = 5 each). In the first branchial arch, lacZ-positive cells are seen in the surface epithelium and mesenchyme in an E9.5 P0-Cre/R26R embryo (a). This distribution shows a strong resemblance to the PDGFRα protein expression pattern seen in an E9.5 wild-type embryo (b). In the frontonasal process in an E9.5 P0-Cre/R26R embryo, lacZ-positive cells are seen in the outer layer of the surface ectoderm and mesenchyme tissue layer (c). Separated lacZ-positive cells are scattered in the neuroepithelial wall of the telencephalon. This distribution shows a strong resemblance to the PDGFRα protein expression pattern in an E9.5 wild-type embryo (d). FNP: frontonasal process, se: surface epithelium, m: mesenchyme, mnc: migratory neural crest, ml: mesenchyme layer, ol: outer layer of the surface ectoderm