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Table 1 Cofactor candidate list and RNAi reporter screen results.

From: Identification of common and cell type specific LXXLL motif EcR cofactors using a bioinformatics refined candidate RNAi screen in Drosophila melanogastercell lines

Well ID

Symbol

Full Name

FBGN#

EcR Bound

Well ID

Symbol

Full Name

FBGN#

EcR Bound

A01

Dfd*

Deformed

FBgn0000439

No

E01

CG5366~

CG5366

FBgn0027568

No

A02

babo~ +

Baboon

FBgn0011300

Yes

E02

MED16~ ^

Mediator complex subunit 16

FBgn0034707

No

A03

E2f2~ +

E2F transcription factor 2

FBgn0024371

Yes

E03

CG5899*

CG5899

FBgn0032157

Nd

A04

ptc~

Patched

FBgn0003892

No

A06

brm~ +

Brahma

FBgn0000212

Yes

A05

mys*~

myospheroid

FBgn0004657

No

E05

abo*

abnormal oocyte

FBgn0000018

No

A06

brm~ +

brahma

FBgn0000212

Yes

E06

HDAC6*

HDAC6

FBgn0026428

Yes

A07

N/A

No RNAi

#N/A

Nd

E07

Rfx+ ^

Rfx

FBgn0020379

No

A08

Hsp27^

Heat shock protein 27

FBgn0001226

No

E08

Taf2+ ^

TBP-associated factor 2

FBgn0011836

No

A09

Hr39

Hormone receptor-like in 39

FBgn0010229

No

E09

CG7154*

CG7154

FBgn0031947

No

A10

Kr ~ +

Kruppel

FBgn0001325

Nd

E10

Acp36DE

Accessory gland peptide 36DE

FBgn0011559

Yes

A11

Myb* ^

Myb oncogene-like

FBgn0002914

No

E11

mus304* +

mutagen-sensitive 304

FBgn0002901

No

A12

tst* ~ + ^

twister

FBgn0039117

Yes

E12

RecQ4*

RecQ4

FBgn0040290

Yes

B01

mia* ^

meiosis I arrest

FBgn0014342

No

F01

Orc5+ ^

Origin recognition complex subunit 5

FBgn0015271

No

B02

neb* ^

nebbish

FBgn0004374

Yes

F02

Sima

Similar

FBgn0015542

No

B03

eIF5B*

eIF5B

FBgn0026259

Yes

F03

MED17

Mediator complex subunit 17

FBgn0038578

No

B04

ida

imaginal discs arrested

FBgn0041147

Nd

F04

MED24

Mediator complex subunit 24

FBgn0035851

No

B05

CG11403*

CG11403

FBgn0026876

No

F05

Su(z)12*

Su(z)12

FBgn0020887

No

B06

CG11970* ^

CG11970

FBgn0027503

No

F06

Mi-2

Mi-2

FBgn0013591

No

B07

MED14*

Mediator complex subunit 14

FBgn0035145

No

F07

Tfb1

Tfb1

FBgn0033929

No

B08

tai* ^

taiman

FBgn0041092

Yes

F08

boss+ ^

bride of sevenless

FBgn0000206

No

B09

zfh2* ^

Zn finger homeodomain 2

FBgn0004607

Yes

F09

Jhe

Juvenile hormone esterase

FBgn0010052

No

B10

yemalpha

yemanuclein alpha

FBgn0005596

No

F10

CG8443^

CG8443

FBgn0034087

No

B11

Sara^

Smad anchor for receptor activation

FBgn0026369

No

F11

HLH106

Helix loop helix protein 106

FBgn0015234

No

B12

CG1582~ ^

CG1582

FBgn0030246

Yes

F12

Iswi

Imitation SWI

FBgn0011604

No

C01

fz3^

frizzled 3

FBgn0027343

No

G01

Asx*

Additional sex combs

FBgn0000142

No

C02

cnc^

cap-n-collar

FBgn0000338

nd

G02

eIF5* ~ +

eIF5

FBgn0030719

Yes

C03

InR^

Insulin-like receptor

FBgn0013984

Yes

G03

CG9323

CG9323

FBgn0032883

No

C04

mor+ ^

moira

FBgn0002783

Yes

G04

dom*

Domino

FBgn0020306

Nd

C05

sas+ ^

stranded at second

FBgn0002306

No

G05

hb* ^

Hunchback

FBgn0001180

No

C06

ush

u-shaped

FBgn0003963

Yes

G06

eIF3-S10*^

eIF3-S10

FBgn0037249

No

C07

CG2990

CG2990

FBgn0030170

No

G07

skd*

Skuld

FBgn0003415

Nd

C08

CG31212

CG31212

FBgn0086613

Nd

G08

Sin3A*

Sin3A

FBgn0022764

No

C09

kz*

kurz

FBgn0001330

No

G09

Tbp-1* +

Tat-binding protein-1

FBgn0028684

Yes

C10

JhI-1* +

Juvenile hormone-inducible protein 1

FBgn0028426

No

G10

Smr*

Smrter

FBgn0024308

No

C11

MED23+ ^

Mediator complex subunit 23

FBgn0034795

No

G11

MED1*

Mediator complex subunit 1

FBgn0037109

No

C12

kis* ~

kismet

FBgn0001309

No

G12

CSN5*

COP9 complex homolog subunit 5

FBgn0027053

No

D01

Chd1* ^

Chromodomain-helicase-DNA-binding protein

FBgn0016132

Yes

H01

Pros45

Pros45

FBgn0020369

No

D02

l(2)01424^

lethal (2) 01424

FBgn0010488

No

H02

Bx42^

Bx42

FBgn0004856

No

D03

trr

trithorax-related

FBgn0023518

No

H03

l(1)G0168^

lethal (1) G0168

FBgn0027287

Nd

D04

MED15^

Mediator complex subunit 15

FBgn0027592

No

H04

hyd~ ^

hyperplastic discs

FBgn0002431

Yes

D05

Hel89B

Helicase 89B

FBgn0022787

No

H05

Nipped-A

Nipped-A

FBgn0053554

Nd

D06

Bap60^

Brahma associated protein 60 kD

FBgn0025463

No

H06

spen^

split ends

FBgn0016977

Yes

D07

XNP

XNP

FBgn0039338

Yes

H07

RpL7A~

Ribosomal protein L7A

FBgn0014026

No

D08

Pk92B+

Protein kinase at 92B

FBgn0014006

No

H08

alien*~

Alien

FBgn0013746

No

D09

POSH^

Plenty of SH3s

FBgn0040294

No

H09

Hdac3~

Hdac3

FBgn0025825

No

D10

Rbf2+

Retinoblastoma-family protein 2

FBgn0038390

No

H10

EcR ~ +

Ecdysone receptor

FBgn0000546

Yes

D11

CG5205

CG5205

FBgn0038344

No

H11

CG12129^

CG12129

FBgn0033475

No

D12

RfC3* ~ ^

RfC3

FBgn0032244

No

H12

Pc* ~

Polycomb

FBgn0003042

No

  1. Listed in the order of orientation in the 96 well plate assay. Symbols in superscript next to gene symbols summarize the results from our RNAi screen for each cell type; * indicates the cofactor was a hit in D20, ~ indicates the cofactor was a hit in L1, + indicates the cofactor was a hit in Kc, ^indicates the cofactor was a hit in S2. In addition, columns indicating whether candidates are also binding targets for EcR based on published whole genome assays and the average expression level of each gene in the relevant cell types from data extracted from NCBI GEO (normalized expression units). Over 85% of the putative candidates were significant hits in our screen