Figure 5

Impaired Hippo pathway signaling promotes scrib¹ + Raf^gof neoplastic tumor overgrowth. Confocal sections through larval eye/antennal discs at day 5 (A-D) and eye/antennal discs still attached to the brain lobes (bl) at day 8 after egg laying (E-H). Mutant clones were generated with ey-FLP and are positively marked by GFP expression (green, or, in the merges, magenta in A-D and yellow in E-H). Grayscale is β-GAL (A-D), F-actin (A-D) or Elav (E-H). Red is F-actin (E-H). (A, B) Expression of Raf^gof does not alter the normal pattern of ex-lacZ (A) or fj-lacZ (B) expression in the eye disc. (C-D) scrib¹ + Raf^gof tumors ectopically express ex-lacZ (C; arrows) and fj-lacZ (D; arrows) within the eye disc. (E-H) scrib¹ + Raf^gof tumors overgrow and fuse with the brain lobes throughout an extended larval stage of development (E). Knockdown of sd (F), halving the gene dosage of yki (G) or expression of yki^RNAi (H) reduces scrib¹ + Raf^gof tumor overgrowth.