Figure 3
Sd is not required for the cell morphology defects in scrib mutant clones. Cross sections of larval eye discs. Mutant clones (A-C) were generated with ey-FLP and are positively marked by GFP expression (green, or magenta and yellow in the merges). Grayscale is Elav and red is F-actin. A white bar indicates the location of the MF. (A-C) Control eye disc clones, generated using a wild type chromosome with a Flippase recognition target (FRT), show the columnar epithelial structure of the eye disc, with the apically localized nuclei of the developing photoreceptor cells marked by Elav (A). In contrast, scrib1 clones expressing bskDN show a disorganized epithelial structure resulting in Elav positive photoreceptor cell nuclei being mislocalized basally within the epithelium (B). Knockdown of sd in scrib1 clones expressing bskDN does not rescue the mutant cell morphology defects (C).