Figure 6

Pax4 expression in pancreata of wild type, Arx^- , Nkx2.2^-/- and Nkx2.2^-/-/Arx^- at E16.5. (A-D) Pax4-expressing cells are present in normal numbers at E16.5 in pancreata of wild type, Arx^-, Nkx2.2^-/- and Nkx2.2^-/-/Arx^- mice. Notably, no co-expression of Pax4 and ghrelin is detectable in wild type or mutant pancreata. Pictures were taken using confocal microscopy. (E) Quantification of Pax4⁺ cells at E16.5 in pancreata of wild type, Arx^-, Nkx2.2^-/- and Nkx2.2^-/-Arx^- mice (n = 3 pancreas per genotype). Single-factor ANOVA coupled to Newman-Keuls test was applied and p-values < 0,05 were assessed as statistically significant compared to wild type. Statistical significance is achieved with the assumption of a normal distribution. Error bars represent SEM.