Figure 5

Effect of the Sciara tra-2 gene on the somatic development of Drosophila. (A) Size (number of bristles) of the sex comb in the Sotra2 transgenic Drosophila flies. The bars in the histograms represent the 95% confidence limits. The sample size is given in parenthesis underneath the histogram. The full genotype of XX pseudomales is ywTDSo#7/w; tra-2^B/Df(2R)trix,tra-2^-; Tub-GAL80^ts,w⁺/rn-GAL4,w⁺ and that of their brothers is XY males ywTDSo#7/Y; tra-2^B/Df(2R)trix,tra-2^-; Tub-GAL80^ts,w⁺/rn-GAL4,w⁺. The cross to generate these flies was: females ywTDSo#7; tra-2^B/CyO,Cy; Tub-GAL80^ts,w⁺ and males w/Y; Df(2R)trix,tra-2^-/CyO,Cy; rn-GAL4,w⁺. (B) Foreleg basitarsus of wild type female. (C) Foreleg basitarsus of XX pseudomale mutant for tra-2 at 18°C, in which the Sciara Tra-2 is not expressed. (D) Foreleg basitarsus of XX pseudomale mutant for tra-2 at 25°C, in which the Sciara Tra-2 is partially expressed. (E) Enlargement of D. SC stands for sex comb.