Figure 4

Hypaxial epithelial dermomyotome grafted to the epaxial domain does not form chondrocytes. A) Schematic illustration of the grafting procedure. Quail hypaxial dermomyotome (blue) of somite 20/21 was transplanted to the epaxial domain of chick embryo (red). dm, dermomyotome; n, neural tube; sc, sclerotome. B) Whole-mount skeletal staining showed a normal chick scapula (s) in the operated region (right). C) Immunohistochemistry on transverse sections through the operated region. Operated side exhibits a cluster of quail cells (red asterisk) giving rise to muscles and connective tissue. n, neural tube; r, rib; s, scapula. Insert in C) shows higher magnification of the grafted QCPN-positive quail cells (red asterisk).