Granzyme Gis expressed in the two-cell stage mouse embryo and is required for the maternal-zygotic transition

Table 3 The developmental potential of mouse embryos cultured in vitro after microinjection of different concentrations of granzyme G antisense morpholinos into the cytoplasm of one-cell stage fertilized eggs

Dosage of Granzyme G Morpholino	Total embryo treated in one-cell stage^†	No. (%) of survival embryo after treatment	No. (%) of embryos developed to
			2-cell embryo	4-cell embryo	8-cell embryo	Morula embryo	Blastocyst embryo
2 mM	64	31(48.4)	25(80.7)^a	7(22.6)^a	0(0.0)^a	0(0.0)^a	0(0.0)^a
0.2 mM	65	33(50.8)	32(97.0) ^b	29(87.9)^b	25(75.8)^b	24(72.7)^b	11(33.3)^b
0.02 mM	81	45(55.6)	45(100.0)^b	45(100.0)^c	43(95.6)^c	43(95.6)^c	43(95.6)^c
0.002 mM	63	39(61.9)	38(97.4)^b	36(92.3)^bc	34(87.2)^bc	34(87.2)^bc	34(87.2)^c

^† Six experiments were carried out in each group, and 10-15 embryos were used in each experiment.
^abc Different superscripts within a column indicate a statistically significant difference (p < 0.05).

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