Figure 6
From: Production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment
The feature of hEBs-derived erythroid cells. (A) Real-time PCR analysis of the globins gene expression of erythroid cells generated from hEBs after 4, 8, 12 or 16 days of liquid culture. *, P < 0.05 when compared with the average expression of ζ, ε, α, γ globins at day 4 and β globin at day 12. (B) Western blotting analysis of the globins protein expression of erythroid cells generated from hEBs at different day. (C) Functional assays of hEBs-derived erythroid cells. Oxygen dissociation curves of RBCs derived from hEBs, hCB or adult blood.