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Table 3 Stimulation of aggregation by cAMP pulses.

From: KeaA, a Dictyostelium kelch-domain protein that regulates the response to stress and development

Strain

% of isolated cells

Wild-type

20.1 +/- 2.5

Wild-type + cAMP

5.6 +/- 1.6

keaA -

38.6 +/- 3.1

keaA- + cAMP

29.2 +/- 2.7

  1. Wild-type and keaA- cells were washed, ressuspended in phosphate buffer to 1 × 107 cells/ml and starved in liquid suspension for 2 hours. At this point 80 nM of cAMP was added every 6 minutes for 4 hours. The number of isolated cells was counted and the percentage to the total number of cells was calculated.
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BMC Developmental Biology

ISSN: 1471-213X

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