Figure 2

Effects of Dicer1 ablation on adrenal apoptosis. TUNEL assay was performed on sections of adrenals at 14.5 dpc, 16.5 dpc, 18.5 dpc, P0, and P5. Green nuclear staining represents positive signals for fragmented DNA and blue staining was the DAPI nuclear counterstain. Immunofluorescence for CYP21 (magenta) was also performed on adrenal sections to label the adrenal cortex. Higher magnification (4× of the original figure) of the apoptotic cells is shown in the inlets. Scale bars represent 100 μm.