Figure 4

ShRNA-mediated knockdown of RNCR2 in the developing retina. Control shRNA or RNCR2-targeted shRNA construct was electroporated into P0.5 retina and harvested at P7 or P21, dissociated and stained for cell-specific markers. (A) RNCR2 knockdown led to a decrease in Müller glia at P7 and (B) an increase in Müller glia and amacrine cells at P21, *p < 0.05. A significant increase in GFP-positive electroporated cells in the INL was noted (B, inset). (C-D) Section immunohistochemistry of retinas electroporated with either control shRNA or RNCR2-targeted shRNA confirm an increase in Müller glia (white arrowheads) and amacrine cells (yellow arrowheads).