Figure 2

EHD protein expression in adult WT, Ehd1^+/- and Ehd1^-/- male mice. Aliquots of 100 μg tissue lysates derived from seven month old male mice were separated using 7.5% SDS-PAGE and Western blots were performed using antisera raised against EHD proteins as described in Methods. The * denotes bands that bled through from the previous blot following stripping. Differential mobility of Hsc70 may represent tissue specific isoforms. Relative molecular weight (MW) markers are indicated in kiloDaltons (kD). Hsc70 served as a loading control.