Figure 8

Competition experiment for module D probes 4 and 5. I. EMSA competition experiment of module D-probe 4. The gel image was obtained with probe only (lane 1) and with 5 and 15 μg nuclear extract (lane 2, 3) to detect the binding protein. The sequences (wild-type or Pax6.1 binding site mutant) for competition are shown below. Three different amounts (10×, 20× and 50×) of competitor were added to the binding reaction with 15 μg nuclear extract. Lanes 4, 5 and 6 are competition experiments with wild-type competitors at 10×, 20× and 50×, respectively, and lanes 7, 8 and 9 are competition experiments with the Pax6.1 site mutant at 10×, 20× and 50×, respectively. II. EMSA competition experiment of module D-probe 5. The gel image was obtained with probe only (lane 1) and with 5 and 15 μg nuclear extract (lane 2 and 3) to detect the binding protein. The sequences (wild-type or FOX binding site mutant) for competition are shown below. Three different amounts (10×, 20× and 50×) of competitor were added to the binding reaction with 15 μg nuclear extract. Lanes 4, 5 and 6 are competition experiments with wild-type competitors at 10×, 20× and 50×, respectively, and lanes 7, 8 and 9 are competition experiments with the FOX site mutant at 10×, 20× and 50×, respectively.