Figure 1

LM511 and LM332 inhibition reduces tumor invasion in human BCC skin xenografts. A: Hematoxylin and eosin-stained sections of human skin xenografts carrying either control GFP or SHH retrovirus and treated with control IgG, 4c7 antibody against LM511 or BM165 antibody against LM332, as indicated. Images captured at 20x magnification. B: Indirect immunofluoresence for human-specific involucrin (red) confirms the human origin of graft tissue. C: Indirect immunofluoresence for keratin 10 (green) indicates all non-basal cells in graft epidermis. D: DIG RNA in-situ with anti-sense probe to PTCH1 transcript in indicated xenografts. Anti-sense probe signal is seen in all SHH-expressing grafts. Black dotted lines indicate epidermal/dermal boundary. Red arrows indicate DIG signal. D': DIG RNA in-situ with sense probe to PTCH1 transcript in indicated xenografts. Images are 20x magnification. E: Indirect immunofluoresence for Ki67 (red) in indicated graft tissues marks proliferating cells. F: TUNEL-TMR Red staining indicates apoptotic cells in graft tissues. E-F: White dashed lines indicate the epidermal/dermal boundary. Scale bars indicate 100 μm.