Figure 3

Hypoplasia of the optic tectum in dmbx1 morphants at 48 hpf. Gross morphologies of the optic tecta were compared between morphants (C, G, K) and both the un-injected (A) and mMO-injected (E, I, M) embryos. Dorsal view, anterior to the left. Contour of the medial-posterior ridge of the optic tectum is demarcated by red doted lines. The MOs used in each group are shown on the bottom right of the panel. Arrows in A, C, E, G, I, K, M represent the relative position where ~ 1 μm plastic sections were obtained as depicted in B, D, F, H, J, L, N, respectively. Measurements of the cross-sectional tectal area are summarized in the graph (O). Asterisk indicates significant difference (p < 0.05) between the morphant samples and the controls. TeO, optic tectum; CeP, cerebellar plate; mob, medulla oblongata; MO, morpholino; mMO, mismatch morpholino.