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Table 2 Nuclear swelling and polar body formation at 6 h after activation in porcine embryos reconstructed by transferring confluent (CON) or nocodazole-treated (NOC) cells into enucleated M I or M II oocytesa

From: Development of porcine embryos reconstituted with somatic cells and enucleated metaphase I and II oocytes matured in a protein-free medium

      

No. of embryos with

           

Swollen nucleus

       

Condensed chromatin

  

One &

 
  

No. of complexes

  

No

  

One &

   

polar

 

Reci-

  

Fused

No. of embryos

chromatin

  

polar

Total

  

body

Total

pient

Donor

Treated

(%)b

Examined

Surviving

/nucleus

One

Two

body

(%)c

One

Two

(%)c

(%)c

M I

CON

128

77 (60.2)d

37

36

5

12

1

0

13(36.1)

18

0

0(0)

18 (50.0)de

 

NOC

200

58 (29.0)e

32

32

11

7

2

1

10(31.3)

9

0

2 (6.3)

11(34.4)d

M II

CON

75

47 (62.7)d

47

45

1

4

0

0

4 (8.9)

38

2

0(0)

40 (88.9)e

 

NOC

122

41 (33.6)e

41

38

9

7

5

2

14 (36.8)

7

4

4(10.5)

15 (39.5)de

  1. aExperiments were repeated 3 times. bPercentage of complexes treated. cPercentage of embryos surviving. d-eValues with different superscripts are significantly different (P < 0.05).
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BMC Developmental Biology

ISSN: 1471-213X

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