Paralysis and delayed Z-disc formation in the Xenopus tropicalis unc45b mutant dicky ticker

Background The protein components of mature skeletal muscle have largely been characterized, but the mechanics and sequence of their assembly during normal development remain an active field of study. Chaperone proteins specific to sarcomeric myosins have been shown to be necessary in zebrafish and invertebrates for proper muscle assembly and function. Results The Xenopus tropicalis mutation dicky ticker results in disrupted skeletal muscle myofibrillogenesis, paralysis, and lack of heartbeat, and maps to a missense mutation in the muscle-specific chaperone unc45b. Unc45b is known to be required for folding the head domains of myosin heavy chains, and mutant embryos fail to incorporate muscle myosin into sarcomeres. Mutants also show delayed polymerization of α-actinin-rich Z-bodies into the Z-disks that flank the myosin-containing A-band. Conclusions The dicky ticker phenotype confirms that a requirement for myosin-specific chaperones is conserved in tetrapod sarcomerogenesis, and also suggests a novel role for myosin chaperone function in Z-body maturation.


Background
The sarcomere is the fundamental unit of the muscle myofibril, and mediates ATP-dependent contraction and relaxation. While the components of sarcomeres are well understood, the mechanics and sequence of their assembly remain under debate. A key early process in myofibrillogenesis is the polymerization of αactinin-rich Z-bodies into the Z-discs that flank the myosin-containing A-band. In a mature vertebrate sarcomere, Z-discs are held in register with the central M-band by titin filaments tightly associated with filamentous actin [1,2].
Molecular chaperones, which mediate specific protein maturation events, have recently been shown to play important roles in vertebrate myofibrillogenesis. Originally identified as an uncoordinated nematode mutation [3,4], the muscle-specific chaperone unc45b cooperates with hsp90 to fold the N-terminal globular motor (S1) domain of sarcomeric myosins [5][6][7]. Depletion of these gene functions in zebrafish results in absence of integrated myosin in the sarcomere [8][9][10][11], but no effects on Z-disc formation were noted. While in vitro studies of these chaperones in cultured mammalian myoblasts have supported roles in sarcomere formation [6,12], in vivo requirements for these proteins have not been described in vertebrates other than teleost fish.
Vertebrate myofibrillogenesis is readily studied in Xenopus embryos, where most skeletal muscle arises from blocks of mesodermally derived somites running along the flank that eventually contribute to the easilyimaged transparent tadpole tail. Somites form and differentiate in a strict anterior-to-posterior sequence, permitting observation of different stages of muscle formation in a single embryo. Recently, a number of Xenopus tropicalis mutations displaying a range of developmental phenotypes [13][14][15] including defective cardiac muscle formation [16] have been described.
In one of these mutations, dicky ticker (dit mh71 ), embryos are completely paralyzed with no heartbeat. Tail muscle shows decreased birefringence under polarized light, consistent with disruption of sarcomeric structures. While the myofibrillogenesis program initiates and muscle myosin is detectable at early stages, sarcomeric myosin heavy chain (MyHC) staining rapidly decreases. Disorganized sarcomeres bounded by α-actinin-rich structures eventually form, but the transition from Z-bodies to Z-discs is delayed. In the first use of the rapid gynogenesis-based positional cloning strategy developed by Khokha et al, [17] we show here that the dit phenotype is caused by a missense mutation in the myosin chaperone unc45b. These results not only demonstrate a conserved role for this protein in vertebrate myofibrillogenesis, but also suggest a novel function for this chaperone in Z-disc formation, a key early step in sarcomerogenesis.

Results
A Cys to Arg conversion in the UCS domain of unc45b disrupts sarcomerogenesis in dit Dit was first identified in a forward genetic screen for Xenopus tropicalis developmental mutations [13]. Homozygous embryos lack a heartbeat (Additional File 1, Movie S1) and are completely paralysed even after vigorous stimulation (Additional File 2, Movie S2), developing cardiac edema and dying by~stage 45. When visualised under polarized light, dit somites showed a marked reduction in birefringence compared to wild type, suggesting disruption to the normal paracrystalline sarcomere structure (Additional File 3, Figure S1) consistent with defective muscle formation rather than aberrant innervation or metabolism. Phalloidin staining of stage 42 tail muscle likewise showed severe disruption to myofibrils (Additional File 3, Figure S1).
The rapid gynogenetic mapping strategy described in Khokha et al. [17] was used to place dit onto a chromosome and estimate a locus-centromere distance. Briefly, dit and sibling wild type gynogenetic embryos were generated by 'early coldshock' suppression of polar body formation in haploid embryos derived from heterozygous carrier females. Bulk segregant pools of mutant and wild type gynogenotes were then assayed with centromeric SSLPs from the 10 X. tropicalis chromosomes [17]. Linkage was detected to marker 016H09 near the centromere of Chromosome 2/Linkage Group 6, but not to other centromeres ( Figure 1A). An estimate of the mutation-centromere distance was obtained from the fraction of phenotypically mutant embryos observed (35%) in gynogenetic clutches, placing the locus~15 cM from the centromere using the formula assuming complete interference (cM) = 50(1 -(2 × fraction mutant gynogenotes)) [18]. SSLPs from this region of the meiotic map as well as additional bespoke SSLPs and SNPs from identified scaffolds were then used for high resolution mapping on embryos obtained from a conventional cross of polymorphic heterozygous carriers. Analysis of 562 mutant embryos (1124 meioses) placed the mutant locus in a 230 kb interval on scaffold_72 (scaf-fold_72:967475-1197607) ( Figure 1B) containing 4 gene models: ap2b1, pex12, aldh3a1, and the sarcomeric myosin chaperone unc45b, whose loss-of-function phenotype in zebrafish closely resembles dit [9]. Sequence of unc45b from wild type and dit embryos revealed a thymidine to cytidine transition at bp 2335 in the coding sequence, converting Cys 779 to Arg ( Figure  1C). Conserved in both vertebrate and invertebrate unc45b orthologs ( Figure 1D), Cys 779 is in the N-terminal UCS (Unc45 Cro1p She4p) domain important for folding myosin in vitro [5,6]. Whole mount in situ hybridization (WISH) of wild type embryos shows unc45b is expressed throughout the somites and heart at stage 28 ( Figure 2A &2B), becoming further localised to the jaw, branchial arches and body wall muscles by stage 40 (Figure 2C &2D). The mutation in a conserved cysteine residue, expression during muscle development, and similarity of loss-of-function phenotypes in zebrafish combine to suggest unc45b may underlie the dit phenotype.

Z-disc formation is delayed in dit
Myofibrillogenesis in dit was investigated further by confocal microscopy. At stage 28, the earliest at which dit can be identified, wild type and mutant embryos were scored, genotyped, and their sarcomeric structures analysed by immunohistochemistry. The oldest (most anterior) somites of wild type sarcomeres showed MyHC (detected by MHC A4.1025) integrating into evenly spaced A-bands ( Figure 3A). Dit embryos at this early stage displayed MyHC staining in disorderly fibrillar structures at levels comparable to wild type siblings ( Figure 3B), in contrast with the negligible MyHC immunoreactivity observed in zebrafish unc45b and hsp90a mutants [9,10]. Headless myosin species retain the capacity to form thick filaments [19], and it is known that unc45b functions to prevent myosin aggregation [20]. The MyHC staining seen in dit during early myofibrillogenesis may represent transient aggregates of filament intermediates in which head domains remain unfolded and are unable to integrate into the sarcomere.
We then evaluated Z-disc formation in dit embryos; Z-disc defects were not described in the zebrafish chaperone mutants steif and sloth [9,10]. In stage 28 X. tropicalis tadpoles, wild type anterior sarcomeres had formed discrete α-actinin-stained Z-disc structures (Figure 3C), while mutants lacked Z-discs but showed punctate staining consistent with α-actinin-rich Z-body precursors ( Figure 3D). Occasional stretches of regularly spaced staining are seen consistent with the onset of sarcomere formation (arrowhead) similar to the premyofibrils described by Sanger et al. [21]. In more posterior somites, sarcomere formation is at an earlier stage. Figure 3E &2F show the border (arrows) between somites 15 and 16 in tails stained with α-actinin. In wild type ( Figure 3E), Z-discs are beginning to assemble into sarcomeres in the slightly older somite 15 (arrowhead, left) while in the adjacent somite 16 no Z-discs are seen. No organized α-actinin staining is seen in mutant embryos at these stages in somite 15-16 ( Figure 3F).

MyHC is lost and Z-disc formation recovers in dit tadpoles
Later in development (stage 43), wild type sarcomeres are organized into paracrystalline structures with MyHCstained A-bands, α-actinin-stained Z-discs, and titinpositive I-bands in register ( Figure 4A, C, E). Dit tadpole tails at this stage showed no organized MyHC fibrils (Figure 4B), and the level of immunoreactivity was significantly weaker than wild type, more closely resembling the zebrafish myosin chaperone mutations [9,10]. Lower MyHC protein levels in dit were confirmed by western blots of stage 40 and 43 embryos using another pan-sarcomeric MyHC antibody, F59 (Additional File 4, Figure  S2). Sarcomeric MyHC proteins are subject to ubiquitinmediated degradation and autophagy [22][23][24], and it is likely that the misfolded head domains in dit are actively degraded. Interestingly, Z-discs (marked by both α-actinin and titin T12 antibodies) were present in dit tadpole tails at these stages, although these were scattered and severely out of register ( Figure 4D &4F).

Depletion of unc45b phenocopies dit
To confirm that a deficit in unc45b function underpinned the dit phenotype, we injected two non-overlapping antisense morpholino oligonucleotides (AMO) into wild type embryos. Both a translation blocking (ATG-MO) and a splice blocking AMO deleting~2/3 of the mature protein (Splice-MO) resulted in highly significant numbers of paralysed or partially paralysed tadpoles lacking heartbeat (ATG-MO 42/49 (p < 0.01); Splice-MO 32/34 (P < 0.01), compared to 0/52 in control AMO-injected embryos). Confocal analysis revealed that both the ATG-MO and  Splice-MO produced specific defects in sarcomere formation mirroring those in dit ( Figure 5), with reductions in sarcomeric MyHC staining and scattered α-actinin-rich Z-disc-like structures. The similarity of the mutant phenotype to those produced by these two independent knockdown experiments is consistent with dit being a strong hypomorph of unc45b.
Interestingly, WISH analysis shows that dit embryos and unc45b antisense-MO injected embryos express increased levels of unc45b mRNA relative to siblings or control MO-injected controls developed in parallel (Figure 6). In zebrafish steif (unc45b) and sloth (hsp90a) mutants, expression of the mutated genes is also upregulated [9,10]. The chaperone hsp90 regulates its own expression by holding the transcription factor hsf1 in a latent complex, which is released during heat shock or pharmacological stress when unfolded proteins compete for hsp90 binding [25][26][27]. The freed hsf1 in turn directly upregulates hsp90 as well as other stress response chaperones [28]. In the dit and steif mutants, persisting unfolded myosin head domains could likewise bind hsp90 and free a heat shock factor to increase chaperone transcription.

Discussion
While the organization of the mature sarcomere is well understood, the order and mechanism of its assembly remain under debate [1,29]. Our data strongly suggest that Z-disc formation, a key early step in myofibrillogenesis, initially requires the presence of mature sarcomeric MyHC proteins. This observation most closely supports the premyofibril model proposed by Du et al. [30] in which closely spaced α-actinin-rich Z-bodies are initially separated by non-muscle myosin II; non-muscle myosin II is then replaced by sarcomeric MyHC proteins as these premyofibrils mature, coincident with Z-body alignment and polymerization into Z-discs (reviewed by Sanger et al. [29]). While closely spaced α-actininstained Z-bodies are observed in dit sarcomeres, lack of mature sarcomeric MyHC replacement could be responsible for delay of polymerization of these into Z-discs, and Z-disc alignment remains poor. Our data provide less support for the model proposed by Holtzer et al. [31], which hypothesizes that titin 'stitches' together αactinin rich I-Z-I bodies with already formed thick filaments to add sarcomere elements at the ends of Z-disc containing striated myofibrils. This model does not anticipate the presence of the regularly spaced premyofibril-like α-actinin-stained Z-body structures observed in early dit sarcomeres. The molecular mechanism by which sarcomeric MyHC might participate in Z-disc maturation remains to be determined. While a direct interaction seems unlikely, feedback to Z-bodies via correct MyHC interdigitation with thin filaments or even contractile activity are also plausible explanations for the requirement for correct MyHC head domain maturation. Our data also do not rule out the possibility that unc45b functions directly in Z-disc maturation on an unknown protein target; indeed, unc45b protein is known to localize to mature Z-discs in zebrafish [32]. Alternatively, incorrectly folded MyHC heads or  aggregates could interfere with Z-disc formation in the mutant, with this block released following myosin degradation.

Conclusions
The X. tropicalis dicky ticker mutant demonstrates a conserved role for unc45b and sarcomeric myosin-specific chaperone function in tetrapod myofibrillogenesis, extending previous phenotypic analysis in teleost fish. A possible novel role for sarcomeric MyHC integration in Z-disc maturation is suggested by the observed delay in aggregation of α-actinin staining. Dicky ticker is the second X. tropicalis mutation to be cloned, and the first to make use of the rapid gynogenesis-based mapping strategy. Analysis of myofibrillogenesis mutations in X. tropicalis, with its potential for combining genetics with embryological and biochemical tools, promises additional insights into function and regulation of vertebrate muscle assembly.
Whole mount in situ hybridization WISH was performed according to Harland et al. [34]. A 181 bp antisense probe was made from a 1217 bp fragment of unc45b (1444 -2661 bp) cloned into pCRII-TOPO, linearised with HincII and transcribed with Sp6.

Additional material
Additional file 1: dit embryos lack heartbeat. Beating heart at stage 40 in wild type (top) is not seen in dit (bottom).