Table 1 List of primers
S. Nos | Gene | Sequence | Tm | Company | |
|---|---|---|---|---|---|
1 | OCT-4 | FP | 5′-AGCGAACCAGTATCGAGAAC-3′ | 59.6 | Sigma |
RP | 5′-TTACAGAACCACACTCGGAC-3′ | ||||
2 | Sox-2 | FP | 5′-AGCTACAGCATGATGCAGGA-3′ | 59.6 | Sigma |
RP | 5′-GGTCATGGAGTTGTACTGCA-3′ | ||||
3 | Nanog | FP | 5′-TGAACCTCAGCTACAAACAG-3′ | 59.6 | Sigma |
RP | 5′-TGGTGGTAGGAAGAGTAAAG-3′ | ||||
4 | Klf-4 | FP | 5′-TCTCAAGGCACACCTGCGAA-3′ | 59.6 | Sigma |
RP | 5′-TAGTGCCTGGTCAGTTCATC-3′ | ||||
5 | hTERT | FP | 5′-ACCAAGCATTCCTGCTCAAGCTG-3′ | 55.7 | Eurofin |
RP | 5′-CGGCAGGTGTGCTGGACACTC-3′ | ||||
6 | HLA-G | FP | 5′-CTGACCGAGACCTGGGCGGGCT-3′ | 60.8 | Sigma |
RP | 5′-GGCTCCATCCTCGGACACGCCGA-3′ | ||||
7 | IDO | FP | 5′-CCTGAGGAGCTACCATCTGC-3′ | 50.7 | Sigma |
RP | 5′-TCAGTGCCTCCAGTTCCTTT-3′ | ||||
8 | PGE-2 | FP | 5′-ACTCTGGCTAGACAGCGTAA-3′ | 62.8 | Sigma |
RP | 5′-ACCGTAGATGCTCAGGGAC-3′ | ||||
9 | GAPDH | FP | 5′-GAC AAG CTT CCC GTT CTC AG-3′ | 55 | Sigma |
RP | 5′-GAC AAG CTT CCC GTT CTC AG-3′ | ||||