Skip to main content
Fig. 2 | BMC Developmental Biology

Fig. 2

From: Identification of reference genes for gene expression studies among different developmental stages of murine hearts

Fig. 2

Overall abundance of the reference genes during development of mouse heart. (A) The melting curve assays of the 21 candidate reference genes across all samples showed the single peak, indicating the RT-qPCR amplification had good specificity; (B) Expression levels of candidate reference gene expression in all heart samples. (C) The expression variabilities of 6 housekeeping genes at 7 different developmental stages. The plots represented the gene expression level of each candidate reference gene in heart samples (n = 38). Values are given as cycle threshold values (Ct values), mean and standard deviation of the Ct values were indicated in the plot

Back to article page