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Fig. 7 | BMC Developmental Biology

Fig. 7

From: Development of the indirect flight muscles of Aedes aegypti, a main arbovirus vector

Fig. 7

Myofibrils and sarcomere maturation during pupae and adult stage. The maturation process of myofibrils and sarcomeres was analyzed by confocal microscopy, staining F-actin with rhodamine-phalloidin (red) and nuclei with DAPI (blue). a In late pupal stage, the myofibrils had a syncytial structure, with nuclei showing a relaxed chromatin organization (white arrows). b In adults, uniform mature myofibrils containing long and organized mature sarcomeres were found. In both pupae and adult IFMs, the nuclei forming rows were observed. c The isolated myofibrils showing the classical structure of mature sarcomere, including the bright Z disc and the dark M-line (magenta and yellow arrowheads). d Magnified image of sarcomere structure. Scale bars: ac 50 µm; d 10 µm

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