Table 2 qRT-PCR primers
Gene | P | Reference | Sequence (5′-3′) | Accession No. |
|---|---|---|---|---|
Hprt | 5’ | MGH PrimerBank | TCAGTCAACGGGGGACATAAA | NM_013556 |
Hprt | 3’ | MGH PrimerBank | GGGGCTGTACTGCTTAACCAG | NM_013556 |
Gli1 | 5’ | MGH PrimerBank | CCAAGCCAACTTTATGTCAGGG | NM_010296 |
Gli1 | 3’ | MGH PrimerBank | AGCCCGCTTCTTTGTTAATTTGA | NM_010296 |
Ptch1 | 5’ | MGH PrimerBank | GCCTTCGCTGTGGGATTAAAG | NM_008957 |
Ptch1 | 3’ | MGH PrimerBank | CTTCTCCTATCTTCTGACGGGT | NM_008957 |
Wdpcp | 5’ | MGH PrimerBank | GCTTGACTGAACTACACCTGTG | NM_145425 |
Wdpcp | 3’ | MGH PrimerBank | TGAGTGTCCAAGGATAATCTCGT | NM_145425 |
Ccnd1 | 5’ | Lopez rios 2012 | CAGACGTTCAGAACCAGATTC | NM_007631 |
Ccnd1 | 3’ | Lopez rios 2012 | CCCTCCAATAGCAGCGAAAAC | NM_007631 |
Sdc3 | 5’ | MGH PrimerBank | AGAGGCCGGTGGATCTTGA | NM_011520 |
Sdc3 | 3’ | MGH PrimerBank | CTCCTGCTCGAAGTAGCCAGA | NM_011520 |
Cdh2 | 5’ | MGH PrimerBank | AGCGCAGTCTTACCGAAGG | NM_007664 |
Cdh2 | 3’ | MGH PrimerBank | TCGCTGCTTTCATACTGAACTTT | NM_007664 |
Col2a1 | 5’ | MGH PrimerBank | GGGAATGTCCTCTGCGATGAC | NM_031163 |
Col2a1 | 3’ | MGH PrimerBank | GAAGGGGATCTCGGGGTTG | NM_031163 |
Sox9 | 5’ | MGH PrimerBank | AGTACCCGCATCTGCACAAC | NM_011448 |
Sox9 | 3’ | MGH PrimerBank | ACGAAGGGTCTCTTCTCGCT | NM_011448 |
Runx2 | 5’ | MGH PrimerBank | CCGCCTCAGTGATTTAGGGC | NM_009820 |
Runx2 | 3’ | MGH PrimerBank | GGGTCTGTAATCTGACTCTGTCC | NM_009820 |
Sp7 | 5’ | MGH PrimerBank | ATGGCGTCCTCTCTGCTTG | NM_130458 |
Sp7 | 3’ | MGH PrimerBank | TGAAAGGTCAGCGTATGGCTT | NM_130458 |
Bglap2 | 5’ | MGH PrimerBank | CTGACCTCACAGATCCCAAGC | NM_001032298 |
Bglap2 | 3’ | MGH PrimerBank | TGGTCTGATAGCTCGTCACAAG | NM_001032298 |
Col1a1 | 5’ | MGH PrimerBank | TAAGGGTCCCCAATGGTGAGA | NM_007742 |
Col1a1 | 3’ | MGH PrimerBank | GGGTCCCTCGACTCCTACAT | NM_007742 |