Fig. 4

Loss of Wdpcp disrupts initiation of chondrogenesis. A, B Alcian Blue staining of day 3 E11.5 control and Wdpcp-cKO forelimb bud micromass cultures treated with hedgehog stimulating chondrogenic differentiation medium. C Expression of hedgehog reporter genes Gli1 and Ptch1 in control versus Wdpcp-cKO forelimb bud micromass cultures. D Expression of proliferation reporter gene Ccnd1 in day 3 forelimb bud micromass cultures. E Expression of early pre-chondrogenic cellular condensation marker N-cadherin (Cdh2), early chondrogenic marker syndecan 3 (Sdc3), and chondrogenic differentiation markers Sox9 and Col2a1 in control versus Wdpcp-cKO day 3 forelimb bud cultures. Scale bar represents 200 μm in (A, B). Error bars represent one standard deviation. Control versus Wdpcp-cKO expression values were compared using Student’s t-test with * for p < 0.05