Fig. 3

Wdpcp-cKO embryos have disrupted hedgehog signaling in developing limb bud and growth plate, and Wdpcp-cKO mesenchymal progenitors show diminished response to hedgehog stimulation. A, B Gli1-Lacz reporter mice were used to observe hedgehog signaling activity (blue) in Wdpcp-cKO versus control E11.5 limb buds. C, D Immunohistochemistry of P0 growth plates for hedgehog signaling reporter Gli1 in Wdpcp-cKO versus control. E, F Expression of hedgehog reporter genes Gli1 and Ptch1 in mesenchymal progenitor cells from Wdpcp-cKO embryos with increasing dosage of smoothened agonist (SAG). Error bars represent one standard deviation. G-J EdU staining for proliferative cells (red) in mesenchymal progenitor cells from Wdpcp-cKO and control with and without SAG treatment. Scale bars represent 200 μm in (A, B) and 50 μm in (C, D); white scale bar represents 50 μm in G-J