Fig. 1
Wdpcp is highly expressed in musculoskeletal tissues and loss of expression in limb bud mesenchyme is associated with ossification, patterning, and growth defects. A RT-PCR analysis of Wdpcp expression in several adult tissues. B Expression of Wdpcp RNA transcripts and (C) Wdpcp protein expression in E11.5 limb buds from Prx1-Cre;WdpcpFlox/+ (control) and Prx1-Cre;WdpcpFlox/Cys40 (Wdpcp-cKO) mice. D, E E17.5 forelimbs stained with Alizarin Red and Alcian Blue showing areas of ossification (red) and cartilaginous condensation (blue) as well as gross skeletal phenotype of Prx1-Cre;WdpcpFlox/+ (control) and Prx1-Cre;WdpcpFlox/Cys40 (Wdpcp-cKO) mice. Black scale bars represent 2 mm in all panels