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Fig. 5 | BMC Developmental Biology

Fig. 5

From: Knockdown of hspg2 is associated with abnormal mandibular joint formation and neural crest cell dysfunction in zebrafish

Fig. 5

The number of Sox10+ cells is increased in morphants at 3 DPF. a-b Tg(sox10:TagRFP) random control (RC) and morphant (MO) larvae (N = 10 per group) were mounted in agarose and confocal images were taken at 3 days post fertilization (DPF). c shows the representative region where cells were quantified with a corresponding schematic showing the parameters (3 rows left, 5 rows right). c′ Average number of Sox10+ cells counted across both groups (N = 10 per group) at 3 DPF. P-value pertains to the statistically significant difference between the RC group and the MOs (*p = 0.04). d qPCR expression of sox10 (N = 24 total) in RC and MO groups at 3 DPF (***p = 0.0005). qPCR was performed on two independent occasions, each represented by a gray dot. Each biological replicate had a minimum of 12 larvae/group for a total n = 24

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