Fig. 2

nkx3.2 expression is decreased in hspg2 morphants. a-b Whole mount in situ hybridization (ISH) was performed to detect the expression of nkx3.2 at the 2 days post fertilization (DPF) stage. Embryos were injected as described in the methods section and subjected to ISH to detect nkx3.2 expression in the developing jaw joint region. Black arrows indicate the expression of nkx3.2. There were N = 18 in the random control (RC) group and N = 14 larvae in the hspg2 morphants (MO) group. a’-b′ shows regions of nkx3.2 expression. Number one and attached arrow indicate the first pharyngeal arch and number five and attached arrow indicate the fifth pharyngeal arch. c qPCR was performed to detect the expression of nkx3.2 at 4 DPF on two independent occasions, each represented by a gray dot. Each biological replicate had a minimum of 7 larvae/group and a total n = 15 across both replicates. **p = 0.004