Fig. 8

Cardiac fibrosis in ANP and DN-FOXO treated hearts following MI-induced cardiac injury. a Schematic of ANP and DN-FOXO treatment after MI. b Cardiac fibrotic tissues were visualized using Masson’s trichrome stain on heart ventricular sections 14 days after MI. Serial sections were cut at 500-μm intervals from the size of the ligature toward the apex. Scale bars: 2000 μm. c Quantification of fibrotic areas in heart sections. d Quantification of relative cardiomyocyte size calculated as cell area from heart section images of co-immunostaining for cTnT and WGA using Image J software. e Cardiomyocyte boundaries were visualized using WGA staining (white) and co-immunostaining with the antibody against cardiac troponin T (cTnT, red) in sectioned heart ventricles. Cell nuclear were stained with DAPI (blue). P value was calculated using one-way ANOVA. **P < 0.01; n.s. = Not significant