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Fig. 2 | BMC Developmental Biology

Fig. 2

From: Natriuretic peptides and Forkhead O transcription factors act in a cooperative manner to promote cardiomyocyte cell cycle re-entry in the postnatal mouse heart

Fig. 2

A temporal natriuretic peptide signal in mouse hearts during early postnatal life. a and b qRT-PCR analysis showing mRNA levels of Anp (a) and Bnp (b) in neonatal heart ventricles at indicated time points. c and d Expression of Anp (c) and Bnp (d) was visualized in ventricular myocardial tissues using in situ hybridization. Scale bars: 500 μm (upper images), 10 μm (lower images). e and f Protein levels of ANP (e) and BNP (f) in heart ventricles and blood serum (plasma) using ELISA analysis. g-i qRT-PCR analysis showing mRNA levels of Npr1 (g), Npr2 (h), and Npr3 (i) in neonatal heart ventricles at indicated time points. P value was calculated using one-way ANOVA. *P < 0.05; ***P < 0.001; ****P < 0.0001; n.s.= Not significant

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