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Fig. 1 | BMC Developmental Biology

Fig. 1

From: Natriuretic peptides and Forkhead O transcription factors act in a cooperative manner to promote cardiomyocyte cell cycle re-entry in the postnatal mouse heart

Fig. 1

Mouse hearts show evidence for cardiomyocyte cell cycle reactivation during early postnatal life. a Cardiomyocytes in the DNA synthesis-phase were visualized by confocal microscope using Click-iT EdU Alexa Fluor (red) and co-immunostaining with antibody against cardiac troponin T (cTnT, green) in sectioned heart left ventricles at indicated postnatal age. Cardiomyocyte boundaries were visualized using WGA staining (white). Cell nuclear were stained with DAPI (blue). Arrows point to EdU+cTnT+ cells. Scale bars: 10 μm. b Quantification of EdU+cTnT+ cells as percentage of total cTnT+ cells (~ 1200 cTnT+ cells per sample). c Confocal image showing cardiomyocytes in mitotic phase detected by co-immunostainings for phosphorylated histone H3 (PH3, red) and cTnT (green) on heart left ventricular tissue sections. d Quantification of PH3+cTnT+ cells as a percentage of total cTnT+ cells analyzed per field. Arrows point to PH3+cTnT+ cells. e and f Percentage of bi-nuclear e and mono-nuclear f cardiomyocytes (CM) in the heart ventricles of neonatal mice (n = 6 to 8 for all analyzed time points). Data are mean ± s.e.m. P value was calculated using Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001. n.s.=not significant

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BMC Developmental Biology

ISSN: 1471-213X

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