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Fig. 5 | BMC Developmental Biology

Fig. 5

From: Interleukin-6 promotes primitive endoderm development in bovine blastocysts

Fig. 5

JAK2 inhibition reduces PE cell numbers. Either regular blastocysts collected on day 7 post-fertilization or expanded blastocysts collected on day 8 were exposed to 0 (DMSO only) or 3 μM AZD1480 for either 24 or 48 h then were fixed and immunostained for PE (GATA6+), EPI (NANOG+) and dual-positive UN cells (NANOG+/GATA6+) (n = 14–25 blastocysts/group over 3 replicate studies). The panels indicate total ICM (Panel a), PE (Panel b), EPI (Panel c), UN (Panel d), and TE cell numbers (Panel e) of day 7 and 8 blastocysts exposed to treatment for 24 or 48 h. Presence of an asterisk indicates a difference between AZD1480 treated and non-treated groups within each day and duration of treatment exposure (P < 0.05)

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