Fig. 4
From: Interleukin-6 promotes primitive endoderm development in bovine blastocysts
IL6 preferentially stimulates pSTAT3Y705 nuclear localization in PE cells. Day 9 blastocysts were treated with 100 ng/ml IL6 for 30 min before fixation and fluorescent immunostaining. Panel a: Example of a blastocyst immunostained for pSTAT3Y705 (red) and NANOG (blue). Dual-positive nuclei will be purple. Panel b: Number and percentage of blastocysts with either some or all ICM nuclei staining for both NANOG and pSTAT3Y705 (n = 24 pSTAT3Y705 positive blastocysts over 4 replicate studies) Panel c: Example of a blastocyst immunostained for pSTAT3Y705 (green) and GATA6 (red). Dual-positive nuclei will be yellow. Panel d: Number and percentage of blastocysts with either some or all ICM nuclei staining for both GATA6 and pSTAT3Y705 (n = 26 pSTAT3Y705 positive blastocysts over 3 replicate studies)