Fig. 2
From: Interleukin-6 promotes primitive endoderm development in bovine blastocysts
Changes in ICM cell populations over time in culture. Blastocysts were cultured in SOF-BE1 until day 8, 9 or 10 post-fertilization, then immunofluorescence staining for PE (GATA6+), EPI (NANOG+) and TE (CDX2+) was completed. Panel a: Example of a day 8 blastocyst immunostained for GATA6 (red), NANOG (blue) and/or CDX2 (green). A higher magnification of the ICM is also shown for the triple-stained image. Cells positive for both GATA6 and NANOG were considered UN cells (purple). Panel b: Analysis of TE, ICM, PE, EPI and UN cell numbers over time (n = 16, 51, and 40 total blastocysts from day 8, 9 and 10, respectively; 3 replicate studies). Different superscripts indicate differences for each cell type (P < 0.05)