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Fig. 5 | BMC Developmental Biology

Fig. 5

From: Dynamic conversion of cell sorting patterns in aggregates of embryonic stem cells with differential adhesive affinity

Fig. 5

Polarity markers and differential distribution of cellular F-actin in ES cell aggregates. a Representative confocal immunofluorescence images of 2-day cultured aggregates from mixture of WT and E-cadherin null ES cells analyzed for E-cadherin and tight junction dependent classical apical polarity markers including ZO-1, aPKC, and Ezrin, and countered stained with DAPI. b Representative confocal immunofluorescence images of mature, 2-day cultured wild-type cell aggregates analyzed for E-cadherin, beta-Actin, and countered stained with DAPI. An arrow indicates the presence of apical actin staining on the surface of the spheroid. c Representative confocal immunofluorescence images of 2-day cultured E-cadherin null ES cell aggregates analyzed for E-cadherin, beta-Actin, and countered stained with DAPI. An arrowhead indicates that the actin staining of surface cells distributes rather uniformly around cell border, lacking polarity. d Two examples of spheroids derived from heterotypic mixing of wild-type and E-cadherin (−/−) ES cells were analyzed for confocal immunofluorescence staining of E-cadherin and actin, counter stained with DAPI. An arrow indicates the surface area that is E-cadherin-positive and shows a polarized actin cap. An arrowhead indicates surface region that is composed of E-cadherin null cells and contains uniformly distributed actin

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