Fig. 4

Transition of sorting patterns. Mixtures of GFP-labeled wild-type and unlabeled wild-type or E-cadherin deficient ES cells were cultured in suspension for 24-h to produce aggregates. Individual spheroids were then imaged by time-lapse video microscopy for another 24 to 48 h. a Serial images at about 3-h intervals were captured from a time-lapse movie of a representative heterotypic aggregate (starting at 24 h), composed of GFP-labeled wild-type and unlabeled E-cadherin (−/−) cells. Images of both GFP alone and overlaid on bright field are shown. b Serial images of an aggregate (starting at 24 h) composed of GFP-labeled and unlabeled wild-type ES cells are shown as a control. The time-lapse movies are included as supplementary data (Supplemental movie 7, 8)