Table 1 Oligonucleotides used in this study
From: Generation and identification of a conditional knockout allele for the PSMD11 gene in mice
Name | Sequence | Purpose |
|---|---|---|
Region 1-F Region 1-R Region 2-F Region 2-R Neo-F Neo-R Region 3-F Region 3-R Region 4-F Region 4-R CRE-F CRE-R F1 R1 F2 R2 F3 S1 S2 S3 S4 GAPDH-F GAPDH-R PSMD11-F PSMD11-R Flp-F Flp-R Probe for neo | 5′-tccatgtctgggctctctactgtttc-3′ 5′-gctgaccgcttcctcgtgcttta-3′ 5′-tatgtctgtgagggtgttggatac-3′ 5′-cagggatccaacatttctga-3′ 5′-tcatctcaccttgctcctgc-3′ 5′-aaggcgatagaaggcgatgc-3′ 5′-tatgtctgtgagggtgttggatac-3′ 5′-cagggatccaacatttctga-3′ 5′-tagatagtgcttccagttatgggag-3′ 5′-gactgttacctacaccatactgctg-3′ 5′-atttgcctgcattaccggtc-3′ 5′-atcaacgttttcttttcgg-3′ 5′-tatgtctgtgagggtgttggatac-3′ 5′-cagggatccaacatttctga-3′ 5′-tagatagtgcttccagttatgggag-3′ 5′-gactgttacctacaccatactgctg-3′ 5′-gttagttggaagaaggtaagggc-3′ 5′-attcgccaatgacaagacgc − 3′ 5′-aacttatagggatttgcctgctacttt-3′ 5′-aaaagaactctagggttgc-3′ 5′-aatctgcctgcctctgcctcccatgtgc-3′ 5′-aaaagggtcatcatctccg-3′ 5′-agtcttctgagtggcagtgat-3′ 5′-agagcagagcatccttgaact-3′ 5′-tcggcagattactcagagcat-3′ 5′-cactgatattgtaagtagtttgc-3′ 5′-ctagtgcgaagtagtgatcagg-3′ 5′-aaggcgatagaaggcgatgcgctgcgaatcgggagcggcgataccgtaaagc acgaggaagcggtcagcccattcgccgccaagctcttcagcaatatcacgggtagc caacgctatgtcctgatagcggtccgccacacccagccggccacagtcgatgaatccagaaaagcggccattttccaccatgatattcggcaagcaggcatcgccatgggtcacgacgagatcatcgccgtcgggcatgcgcgccttgagcctggcgaacagttcggctggcgcgagcccctgatgctcttcgtccagatcatcctgatcgacaagaccggcttccatccgagtacgtgctcgctcgatgcgatgtttcgcttggtggtcgaatgggcaggtagccggatcaagcGtatgcagccgccgcattgcatcagccatgatggatactttctcggcaggagcaaggtgagatga-3’ | Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Gene targeting Sequencing Sequencing Sequencing Sequencing qRT-PCR qRT-PCR qRT-PCR qRT-PCR Gene targeting Gene targeting Southern blot |