Fig. 3

Floxed PSMD11 allele could be deleted efficiently in vitro with Cre-mediated gene recombination. a No difference of PSMD11 expression could be found in liver and pancreas between adult WT and PSMD11^flx/flx mice by western blot. n = 3 each group. b qRT-PCR to detect PSMD11 mRNA expression in RNA from adult WT and PSMD11 ^flx/flx mouse liver and pancreas tissues, n = 3 each group. c PCR analysis of DNA from AAV (expressing ZsGreen or Cre) infected PSMD11 ^flx/flx MEFs and a nonrecombined PSMD11 ^flx/+ mouse without Cre expression (NC), primer F3 and R1 will produce 643 bp bands in PSMD11 exon 5 deleted alleles, primers F2 and R2 will produce 463 bp and 335 bp bands in PSMD11 exon 5 floxed or WT alleles respectively. d Quantification of PSMD11 mRNA by real-time RT-PCR on total RNA extracted from AAV (expressing ZsGreen or Cre) infected PSMD11 ^flx/flx MEFs. e Western blot to show nearly complete depletion of PSMD11 protein in Cre-infected PSMD11 ^flx/flx MEFs. f Quantification of PSMD11 protein expression in e which was normalized to β-Actin and expressed as percent of the controls. The mean ± SEM of two independent experiments was shown. **P < 0.01, *P < 0.05